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glut 4  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc glut 4
    Glut 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+glut+4/10__1096_slash_fj__202504597r-133-23-7
    Average 86 stars, based on 1 article reviews
    glut 4 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Saline:

    Article Title: Sustained elevation of MG53 in the bloodstream increases tissue regenerative capacity without compromising metabolic function
    Article Snippet: Heart, muscle lysates or serum samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene fluoride membranes (PVDF) (Millipore). .. The blots were washed with Tris-buffered saline Tween-20 (TBST), blocked with 5% milk in TBST for 1 h, and incubated with custom-made monoclonal anti-MG53 antibody (1:2000) , or commercial IRS-1 antibody (Invitrogen, Cat. No. 700662, 1:1000), anti-PPARα antibody (NovusBio, Cat. No. NB600-636, 1:1000), anti-IR-β antibody (Cell Signaling, Cat. No. 3025, 1:1000), and anti-Glut-4 (Cell Signaling, Cat. No. 2213, 1:1000) antibody. .. Immunoblots were visualized with an ECL plus kit (Pierce).

    Article Title: Sustained elevation of MG53 in the bloodstream increases tissue regenerative capacity without compromising metabolic function.
    Article Snippet: Heart, muscle lysates or serum samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene fluoride membranes (PVDF) (Millipore). .. The blots were washed with Tris-buffered saline Tween-20 (TBST), blocked with 5% milk in TBST for 1 h, and incubated with custom-made monoclonal anti-MG53 antibody (1:2000)18,30 or commercial IRS-1 antibody (Invitrogen, Cat. No. 700662, 1:1000), anti-PPARα antibody (NovusBio, Cat. No. NB600-636, 1:1000), anti-IR-β antibody (Cell Signaling, Cat. No. 3025, 1:1000), and anti-Glut-4 (Cell Signaling, Cat. No. 2213, 1:1000) antibody. .. Immunoblots were visualized with an ECL plus kit (Pierce).

    Incubation:

    Article Title: Sustained elevation of MG53 in the bloodstream increases tissue regenerative capacity without compromising metabolic function
    Article Snippet: Heart, muscle lysates or serum samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene fluoride membranes (PVDF) (Millipore). .. The blots were washed with Tris-buffered saline Tween-20 (TBST), blocked with 5% milk in TBST for 1 h, and incubated with custom-made monoclonal anti-MG53 antibody (1:2000) , or commercial IRS-1 antibody (Invitrogen, Cat. No. 700662, 1:1000), anti-PPARα antibody (NovusBio, Cat. No. NB600-636, 1:1000), anti-IR-β antibody (Cell Signaling, Cat. No. 3025, 1:1000), and anti-Glut-4 (Cell Signaling, Cat. No. 2213, 1:1000) antibody. .. Immunoblots were visualized with an ECL plus kit (Pierce).

    Article Title: Icariin treatment reduces blood glucose levels in type 2 diabetic rats and protects pancreatic function
    Article Snippet: .. Subsequently the membranes were incubated with anti-GLUT-4 (2213S, Cell Signaling Technology, America), anti-phospho (p)-AMPK (2535S, Cell Signaling Technology), total-AMPK (2532S, Cell Signaling Technology) or anti-β-actin (ab8227, Abcam). .. Membranes were then incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies, including HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) (AP132P, Merck Millipore), or HRP-conjugated goat anti-mouse IgG (ab6789, Abcam).

    Article Title: Treatment with crocin attenuates cardiac metabolic disturbances and subsequent inflammation in streptozotocin-induced diabetes
    Article Snippet: Equal amounts of protein were separated on 10% (w/v) acrylamide and 0.275% (w/v) bis-acrylamide gels and transferred onto 0.45 μm nitrocellulose membranes (Schleicher and Schuell, Keene, USA). .. Subsequently, the membranes were incubated overnight with the appropriate primary antibodies: anti-PPARδ (#101720, Cayman), anti-PPARα (#101710, Cayman), anti-phospho Akt (#9271, Cell Signaling), anti-Akt (#4691, Cell Signaling), anti-Glut-4 (#2213, Cell Signaling), anti-Na-K ATPase (#3010, Cell Signaling), anti-IL-6 (#CSB-PA06757 A0Rb, Cusabio), anti-TNFα (#CSB-PA07427 A0Rb, Cusabio), anti-α-tubulin (#2125, Cell Signaling) and anti-β-actin (#3700, Cell Signaling). .. After washing in TBST (3x5 min) the blots were incubated with horseradish peroxidase-linked secondary antibodies, washed again in TBST (3x5 min), and the bands were detected using enhanced chemiluminescence (Cell Signaling, Boston, USA) with exposure to Fuji Medical X-ray films.

    Article Title: Hydroalcoholic Extracts of Cucumis prophetarum L. Affect the Insulin Signaling Pathway in an In Vitro Model of Insulin-Resistant L6 Myotubes.
    Article Snippet: .. The membranes were incubated with anti-P-IRS1 (Tyr612) (Invitrogen, Carlsbad, CA, USA), anti-P-IRS1 (Ser307) (Millipore, Burlington, MA, USA), anti-IRS1 (Cell Signaling, Danvers, MA, USA), anti-P-AKT (Ser473) (Cell Signaling), anti-AKT (Cell Signaling), anti-P-GSK3β (Ser9) (ABclonal, Woburn, MA, USA), anti-GSK3β (ABclonal), anti-P-AMPKα (Thr172) (Cell Signaling), anti-AMPKα (Cell Signaling), anti-P-mTOR (Ser2448) (Cell Signaling), antimTOR (Cell Signaling), anti-GLUT-4 (Cell Signaling) and anti-β-Actin (Bioss) antibodies at 4 ◦C overnight. .. As secondary antibodies, peroxidase anti-rabbit IgG (abcam-1:4000 dilution) and peroxidase anti-mouse IgG (abcam-1:4000 dilution) were used.

    Article Title: Treatment with crocin attenuates cardiac metabolic disturbances and subsequent inflammation in streptozotocin-induced diabetes.
    Article Snippet: Equal amounts of protein were separated on 10% (w/v) acrylamide and 0.275% (w/v) bis-acrylamide gels and transferred onto 0.45 μm nitrocellulose membranes (Schleicher and Schuell, Keene, USA). .. Subsequently, the membranes were incubated overnight with the appropriate primary antibodies: anti-PPARδ (#101720, Cayman), anti-PPARα (#101710, Cayman), anti-phospho Akt (#9271, Cell Signaling), anti-Akt (#4691, Cell Signaling), anti-Glut-4 (#2213, Cell Signaling), anti-Na-K ATPase (#3010, Cell Signaling), anti-IL-6 (#CSB-PA06757 A0Rb, Cusabio), anti-TNFα (#CSB-PA07427 A0Rb, Cusabio), anti-α-tubulin (#2125, Cell Signaling) and anti-β-actin (#3700, Cell Signaling). .. After washing in TBST (3x5 min) the blots were incubated with horseradish peroxidase-linked secondary antibodies, washed again in TBST (3x5 min), and the bands were detected using enhanced chemiluminescence (Cell Signaling, Boston, USA) with exposure to Fuji Medical X-ray films.

    Article Title: Sustained elevation of MG53 in the bloodstream increases tissue regenerative capacity without compromising metabolic function.
    Article Snippet: Heart, muscle lysates or serum samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene fluoride membranes (PVDF) (Millipore). .. The blots were washed with Tris-buffered saline Tween-20 (TBST), blocked with 5% milk in TBST for 1 h, and incubated with custom-made monoclonal anti-MG53 antibody (1:2000)18,30 or commercial IRS-1 antibody (Invitrogen, Cat. No. 700662, 1:1000), anti-PPARα antibody (NovusBio, Cat. No. NB600-636, 1:1000), anti-IR-β antibody (Cell Signaling, Cat. No. 3025, 1:1000), and anti-Glut-4 (Cell Signaling, Cat. No. 2213, 1:1000) antibody. .. Immunoblots were visualized with an ECL plus kit (Pierce).

    other:

    Article Title: Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer of glucose uptake and modulator of insulin signaling-associated pathways.
    Article Snippet: Anti-IRS-1 (Cat#2390), anti-phospho-IRS-1 (Ser612; Cat#3203), anti-ACC (Cat#3676), anti-phospho-ACC (Ser79; Cat#3661), anti-Akt (Cat#9272), anti-phospho-Akt (Ser473; Cat#4058), anti-AMPK (Cat#5832), anti-phospho-AMPK (Thr172; Cat#2531), anti-PI3K (Cat#4257), anti-p38 MAPK (Cat#9212), anti-phospho-p38 MAPK (Thr180/Tyr182; Cat#9216), anti-ERK1/2 MAPK (Cat#9102), anti-phospho-ERK1/2 MAPK (Thr202/Tyr204; Cat#9101), anti-GLUT-4 (Cat#2213), HRP-linked anti-mouse IgG (Cat#7076), and HRP-linked anti-rabbit IgG (Cat#7074) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).



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